DNA bar coding and pyrosequencing to identify rare HIV drug resistance mutations

@inproceedings{Hoffmann2007DNABC,
  title={DNA bar coding and pyrosequencing to identify rare HIV drug resistance mutations},
  author={Christopher Hoffmann and Nana K. Minkah and Jeremy N Leipzig and Gary Wang and Max Q. Arens and Pablo Tebas and Frederic D. Bushman},
  booktitle={Nucleic acids research},
  year={2007}
}
Treatment of HIV-infected individuals with antiretroviral agents selects for drug-resistant mutants, resulting in frequent treatment failures. Although the major antiretroviral resistance mutations are routinely characterized by DNA sequencing, treatment failures are still common, probably in part because undetected rare resistance mutations facilitate viral escape. Here we combined DNA bar coding and massively parallel pyrosequencing to quantify rare drug resistance mutations. Using DNA bar… CONTINUE READING