DNA and protein contents of hepatocytes in primary cultures monitored by flow cytometry: Effect of phenobarbital and dimethylsulphoxide.

Abstract

Primary rat hepatocyte cultures were analysed by two-parameter flow cytometry (FCM) analysis. The simultaneous highly reproducible measurements of DNA and protein contents of hepatocytes showed a high resolution and allowed individual ploidy classes to be monitored during culture. Among both the 2N- and 4N-cell populations of freshly isolated hepatocytes… (More)

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@article{Holzer1987DNAAP, title={DNA and protein contents of hepatocytes in primary cultures monitored by flow cytometry: Effect of phenobarbital and dimethylsulphoxide.}, author={C. Holzer and Philipp W. Maier}, journal={Toxicology in vitro : an international journal published in association with BIBRA}, year={1987}, volume={1 4}, pages={203-13} }