DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.

@article{Ou1988DNAAF,
  title={DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells.},
  author={C. Y. Ou and S. Kwok and Steven W. Mitchell and David H. Mack and John Sninsky and John W. Krebs and Paul Feorino and D T Warfield and G. Schochetman},
  journal={Science},
  year={1988},
  volume={239 4837},
  pages={295-7}
}
By means of a selective DNA amplification technique called polymerase chain reaction, proviral sequences of the human immunodeficiency virus (HIV-1) were identified directly in DNA isolated from peripheral blood mononuclear cells (PBMCs) of persons seropositive but not in DNA isolated from PBMCs of persons seronegative for the virus. Primer pairs from multiple regions of the HIV-1 genome were used to achieve maximum sensitivity of provirus detection. HIV-1 sequences were detected in 100% of DNA… CONTINUE READING