• Biology, Medicine
• Published in Journal of bacteriology 1994
• DOI:10.1128/jb.176.11.3224-3230.1994

DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.

@article{Mackay1994DNAAR,
  title={DNA alkylation repair limits spontaneous base substitution mutations in Escherichia coli.},
  author={William J. Mackay and Suhua Han and Leona D. Samson},
  journal={Journal of bacteriology},
  year={1994},
  volume={176 11},
  pages={
          3224-30
        }
}

• William J. Mackay, Suhua Han, Leona D. Samson

The Escherichia coli Ada and Ogt DNA methyltransferases (MTases) are known to transfer simple alkyl groups from O6-alkylguanine and O4-alkylthymine, directly restoring these alkylated DNA lesions to guanine and thymine. In addition to being exquisitely sensitive to the mutagenic effects of methylating agents, E. coli ada ogt null mutants display a higher spontaneous mutation rate than the wild type. Here, we determined which base substitution mutations are elevated in the MTase-deficient cells… CONTINUE READING

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