DNA Topoisomerase VI Generates ATP-dependent Double-strand Breaks with Two-nucleotide Overhangs*

@article{Buhler2001DNATV,
  title={DNA Topoisomerase VI Generates ATP-dependent Double-strand Breaks with Two-nucleotide Overhangs*},
  author={Cyril Buhler and Joyce H. G. Lebbink and Chantal Bocs and Rudolf Ladenstein and Patrick Forterre},
  journal={The Journal of Biological Chemistry},
  year={2001},
  volume={276},
  pages={37215 - 37222}
}
A key step in the DNA transport by type II DNA topoisomerase is the formation of a double-strand break with the enzyme being covalently linked to the broken DNA ends (referred to as the cleavage complex). In the present study, we have analyzed the formation and structure of the cleavage complex catalyzed by Sufolobus shibatae DNA topoisomerase VI (topoVI), a member of the recently described type IIB DNA topoisomerase family. A purification procedure of a fully soluble recombinant topoVI was… Expand
The ancestral role of ATP hydrolysis in type II topoisomerases: prevention of DNA double-strand breaks
TLDR
It is proposed that the energy of ATP hydrolysis is needed to control the separation of protein–protein interfaces and prevent the accidental formation of potentially mutagenic or cytotoxic DSBs. Expand
The structure of DNA-bound human topoisomerase II alpha: conformational mechanisms for coordinating inter-subunit interactions with DNA cleavage.
TLDR
The 2.9-Å-resolution structure of the DNA cleavage core of human topoisomerase IIα (TOP2A) bound to a doubly nicked, 30-bp duplex oligonucleotide is determined and reveals that the principal site of DNA engagement undergoes highly quantized conformational transitions between distinct binding, cleavage, and drug-inhibited states that correlate with the control of subunit-subunit interactions. Expand
SURVEY AND SUMMARY The ancestral role of ATP hydrolysis in type II topoisomerases: prevention of DNA double-strand breaks
TLDR
It is proposed that the energy of ATP hydrolysis is needed to control the separation of protein–protein interfaces and prevent the accidental formation of potentially mutagenic or cytotoxic DSBs. Expand
Gyrase containing a single C-terminal domain catalyzes negative supercoiling of DNA by decreasing the linking number in steps of two
TLDR
Gyrase that contains a single CTD binds, distorts, and cleaves DNA similarly to wildtype gyrase, and shows wildtype-like DNA-dependent ATPase activity, and undergoes DNA-induced movement of the CTD as well as N-gate narrowing. Expand
Crystal structure of an intact type II DNA topoisomerase: insights into DNA transfer mechanisms.
TLDR
This work presents the crystal structure of intact DNA topoisomerase VI bound to radicicol, an inhibitor of human topo II, and compares it to the conformation of the apo-protein as determined by small-angle X-ray scattering in solution to propose a structural model for the two-gate DNA transfer mechanism. Expand
Holoenzyme assembly and ATP-mediated conformational dynamics of topoisomerase VI
TLDR
Small-angle X-ray scattering studies show that nucleotide binding elicits a major structural reorganization that is propagated to the enzyme's DNA-cleavage center, explaining how ATP is coupled to DNA capture and strand scission. Expand
Structure of the topoisomerase VI‐B subunit: implications for type II topoisomerase mechanism and evolution
TLDR
The data demonstrate that type IIA and type IIB topoisomersases have descended from a common ancestor and reveal how ATP turnover generates structural signals in the reactions of both type II topoisomerase families. Expand
Topoisomerase VI senses and exploits both DNA crossings and bends to facilitate strand passage
TLDR
These observations explain how topo VI tightly coordinates DNA crossover recognition and ATP binding with strand scission, providing useful insights into the operation of type IIB topoisomerases and related meiotic recombination and GHKL ATPase machineries. Expand
Direct measurement of DNA bending by type IIA topoisomerases: implications for non-equilibrium topology simplification
TLDR
Data suggest that DNA bending is not the sole determinant of non-equilibrium topology simplification, and suggest a fundamental and conserved role for DNA bending in the enzymatic cycle of type IIA topoisomerases. Expand
New mechanistic and functional insights into DNA topoisomerases.
TLDR
Recent advances in mechanistic insights into topoisomerases are covered, including a DNA helicase capable of modulating the directionality of strand passage, enabling important functions like reannealing denatured DNA and resolving recombination intermediates. Expand
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References

SHOWING 1-10 OF 35 REFERENCES
Single strand DNA cleavage reaction of duplex DNA by Drosophila topoisomerase II.
TLDR
It is shown that under single strand cleavage conditions, topoisomerase II still possesses a low level of double strand passage activity: it can introduce topological knots into both covalently closed or nicked DNA rings, and change the linking number of a plasmid DNA by steps of two. Expand
Double strand DNA cleavage by type II DNA topoisomerase from Drosophila melanogaster.
TLDR
The purified type II DNA topoisomerase from the embryos of Drosophila melanogaster exists in its native form as a dimer of 170,000-dalton polypeptides, which suggests that they share some fundamental features in their mechanism of action. Expand
DNA transport by a type II DNA topoisomerase: Evidence in favor of a two-gate mechanism
TLDR
Results favor a two-gate model in which the DNA ring being transported can exit from the interior of the enzyme through a gate on the opposite side of the entrance gate, which is irreversibly closed upon binding of the nonhydrolyzable ATP analog. Expand
Quaternary changes in topoisomerase II may direct orthogonal movement of two DNA strands
TLDR
A new structure of the Saccharomyces cerevisiae topoisomerase II DNA-binding and cleavage core suggests that in addition to conformational changes in the DNA-opening platform, a dramatic reorganization of accessory domains may occur during catalysis. Expand
Site-specific cleavage of DNA by E. coli DNA gyrase
TLDR
Analysis of cleavage fragments showed that cutting between a TG doublet is common to most, or all, gyrase cleavages, and this diverse recognition sequence with common elements is a pattern shared with several other specific nucleic acid-protein interactions. Expand
Structure and function of an archaeal topoisomerase VI subunit with homology to the meiotic recombination factor Spo11
TLDR
The A subunit of topoisomerase VI is homologous to the meiotic recombination factor, Spo11, and this structure can serve as a template for probing Spo11 function in eukaryotes. Expand
Crystal structure of the breakage–reunion domain of DNA gyrase
DNA gyrase is a type II DNA topoisomerase from bacteria that introduces supercoils into DNA,. It catalyses the breakage of a DNA duplex (the G segment), the passage of another segment (the T segment)Expand
An atypical topoisomerase II from archaea with implications for meiotic recombination
TLDR
It is suggested that Spo11 catalyses the formation of double-strand breaks that initiate meiotic recombination in S. cerevisiae by analogy with the mechanism of action of known type II topoisomerases. Expand
Similarity in the catalysis of DNA breakage and rejoining by type IA and IIA DNA topoisomerases.
  • Q. Liu, J. Wang
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 1999
TLDR
DNA breakage and rejoining by either a type IA or a type IIA enzyme are shown to involve cooperation between a DNA-binding domain containing the active-site tyrosine and a Rossmann fold containing several highly conserved acidic residues. Expand
A topoisomerase from Escherichia coli related to DNA gyrase.
TLDR
Topoisomerase II' resembles gyrase in its sensitivity to oxolinic acid, the wrapping of DNA in an apparent positive supercoil around the enzyme, and the introduction in an aborted reaction of site-specific double-strand breaks in the DNA with concomitant covalent attachment of protein to both newly created 5' ends. Expand
...
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3
4
...