DNA Topoisomerase VI Generates ATP-dependent Double-strand Breaks with Two-nucleotide Overhangs*

@article{Buhler2001DNATV,
  title={DNA Topoisomerase VI Generates ATP-dependent Double-strand Breaks with Two-nucleotide Overhangs*},
  author={Cyril Buhler and Joyce H. G. Lebbink and Chantal Bocs and Rudolf Ladenstein and Patrick Forterre},
  journal={The Journal of Biological Chemistry},
  year={2001},
  volume={276},
  pages={37215 - 37222}
}
A key step in the DNA transport by type II DNA topoisomerase is the formation of a double-strand break with the enzyme being covalently linked to the broken DNA ends (referred to as the cleavage complex). In the present study, we have analyzed the formation and structure of the cleavage complex catalyzed by Sufolobus shibatae DNA topoisomerase VI (topoVI), a member of the recently described type IIB DNA topoisomerase family. A purification procedure of a fully soluble recombinant topoVI was… 

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