DNA Purification

@inproceedings{Herzer2001DNAP,
  title={DNA Purification},
  author={Sibylle U. Herzer},
  year={2001}
}
Materials: • Stratagene StrataPrep DNA Gel Extraction Kit (Catalog #400766 or #400768) http://www.stratagene.com/products/displayProduct.aspx?pid=448 • 0.22um Millipore Ultrafreee -MC Sterile GV Durapore (1.5mL yellow dot tubes with filter cups) (Catalog #UFC30GV0S) (4 labeled: A1, A2, B1, B2) http://www.millipore.com/catalogue/itemdetail.do?id=UFC30GV0S • Plasmid DNA • Appropriate Enzymes & Buffers • DNA Purification Work Sheet • UV illuminator • Gel Photograph Equipment • Agarose… 
A rapid and low-cost DNA extraction method for isolating Escherichia coli DNA from animal stools
TLDR
Results obtained show that, this method using the lysis buffer containing α-casein, produces PCR ready DNA at a fraction of the cost of commercial DNA extraction kits.
Comparative analysis of genomic DNA extraction methods from small ruminant blood and milk cells.
TLDR
Genomic DNA samples from milk have showed the lowest percentage of PCR positive results which suggest the need to include additional protocol steps in order to eliminate putative inhibitors of PCR, and the best extraction method was DNAzol followed by Silica, regardless of the sample type.
Optimization of high molecular weight DNA extraction methods in shrimp for a long-read sequencing platform
TLDR
This study identified an effective extraction method for high-quality HMW genomic DNA of shrimp that can be applied to other marine organisms for a long-read sequencing platform.
Development of a competitive PCR assay for the quantification of total Escherichia coli DNA in water
TLDR
The aim was to investigate the use of c-PCR reaction to detect and quantify, without prior enrichment, Escherichia coli in water samples, and to estimate E. coli numbers that gave comparable results with the culture based microbiological data obtained.
Proteomic analysis of Glossina pallidipes salivary gland hypertrophy virus virions for immune intervention in tsetse fly colonies.
TLDR
Rabbit antibodies produced in rabbits against candidate proteins involved in peroral infectivity of tsetse flies suggest that immune intervention of viral infections in colonies of G. pallidipes is a realistic option.
Detection of diarrhoeagenic Escherichia coli in clinical and environmental water sources in South Africa using single-step 11-gene m-PCR
TLDR
The optimised single-step 11-gene m-PCR reactions could be successfully used for the identification of pathogenic and non-pathogenic E. coli types and showed monitoring for PCR inhibition to ensure correct reporting of the results.
Ancient human microbiomes.
Design & Fabrication of Microfluidic DNA Extraction Device for Water Quality Monitoring
TLDR
This review concludes with a discussion of the author's methodology and its applications in the context of literature and tourism.
Microbiological analysis and control of the fruit vinegar production process
En esta tesis se realizo la identificacion y caracterizacion de los microorganismos (levaduras y bacterias aceticas) asociados a la produccion de vinagres de diferentes frutas con el fin de
...
1
2
...

References

SHOWING 1-10 OF 90 REFERENCES
Rapid isolation of plasmid DNA by LiCl-ethidium bromide treatment and gel filtration.
Preparation of pure plasmid or cosmid DNA using single-strand affinity matrix and gel-filtration spin columns.
TLDR
A rapid method for ultrapure plasmid or cosmid DNA isolation from ten-mL to several hundred-mL cultures of Escherichia coli produces consistent yields of high-quality plasmids that are suitable for use in many molecular biology applications.
Rapid and simple method for purification of nucleic acids
TLDR
A simple, rapid, and reliable protocol for the small-scale purification of DNA and RNA from, e.g., human serum and urine, based on the lysing and nuclease-inactivating properties of guanidinium thiocyanate together with the nucleic acid-binding properties of silica particles or diatoms in the presence of this agent.
A modified alkaline lysis method for the preparation of highly purified plasmid DNA from Escherichia coli.
TLDR
This procedure could fully replace plasmids purified on CsCl gradients for more demanding tasks such as the in vitro synthesis of RNA probes by phage RNA polymerases, the generation of deletion mutants with exonuclease III, and the transfection of mammalian cells by the calcium phosphate coprecipitation method.
Preparation of milligram amounts of 21 deoxyribonucleic acid restriction fragments.
TLDR
A rapid and improved method for the purification of plasmids and DNA restriction fragments was developed and the linear pVH51 vector DNA was digested with HaeIII to yield fragments ranging from 12 to 880 bp.
Preparative and analytical purification of DNA from agarose.
TLDR
Two procedures were developed for removing DNA from agarose after electrophoretic separation of DNA fragments according to size, and the sequence-monitoring system is quantitative, directly measuring the proportion of the probe complementary to a given DNA fragment and vice versa.
Purification of plasmid DNA by tangential flow filtration.
TLDR
A simple, scalable method for purification of plasmid DNA, which has been demonstrated to be effective in the isolation of seven different plasmids and had comparable transfection capability relative to a classical, cesium chloride gradient-based method.
A simple procedure for large-scale preparation of pure plasmid DNA free from chromosomal DNA from bacteria.
...
1
2
3
4
5
...