DNA Fingerprinting of Pearls to Determine Their Origins

  title={DNA Fingerprinting of Pearls to Determine Their Origins},
  author={Joana Beatrice Meyer and Laurent E. Cartier and Eric A. Pinto-Figueroa and Michael S. Krzemnicki and Henry A. H{\"a}nni and Bruce A McDonald},
  booktitle={PloS one},
We report the first successful extraction of oyster DNA from a pearl and use it to identify the source oyster species for the three major pearl-producing oyster species Pinctada margaritifera, P. maxima and P. radiata. Both mitochondrial and nuclear gene fragments could be PCR-amplified and sequenced. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay in the internal transcribed spacer (ITS) region was developed and used to identify 18 pearls of unknown origin… CONTINUE READING
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