DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space

  title={DNA Damage and Survival Time Course of Deinococcal Cell Pellets During 3 Years of Exposure to Outer Space},
  author={Yuko Kawaguchi and Mio Shibuya and Iori Kinoshita and Junichi Yatabe and Issay Narumi and Hiromi Shibata and Risako Hayashi and Daisuke Fujiwara and Yuka Murano and Hirofumi Hashimoto and Ei-ichi Imai and Satoshi Kodaira and Yukio Uchihori and Kazumichi Nakagawa and Hajime Mita and Shin-Ichi Yokobori and Akihiko Yamagishi},
  journal={Frontiers in Microbiology},
The hypothesis called “panspermia” proposes an interplanetary transfer of life. Experiments have exposed extremophilic organisms to outer space to test microbe survivability and the panspermia hypothesis. Microbes inside shielding material with sufficient thickness to protect them from UV-irradiation can survive in space. This process has been called “lithopanspermia,” meaning rocky panspermia. We previously proposed sub-millimeter cell pellets (aggregates) could survive in the harsh space… 

Figures from this paper

Non-random genetic alterations in the cyanobacterium Nostoc sp. exposed to space conditions

The variant profile showed that biofilm and photosystem associated loci were the most altered, with an increased variant rate of synonymous base pair substitutions, and the cause(s) of these non-random alterations and their implications to the evolutionary potential of single bacterial cells under long-term cosmic exposure warrants further investigation.

Investigation of Nostoc sp. HK-01, Cell Survival over Three Years during the Tanpopo Mission.

To the best of the knowledge, this is the first pure strain of Nostoc sp.

Mutation Analysis of the rpoB Gene in the Radiation-Resistant Bacterium Deinococcus radiodurans R1 Exposed to Space during the Tanpopo Experiment at the International Space Station.

The experiment suggests that the dried cells of the microorganism D. radiodurans can travel without space-specific deterioration that may induce excess mutations relative to travel at Earth's surface.

Proteomic Response of Deinococcus radiodurans to Short-Term Real Microgravity during Parabolic Flight Reveals Altered Abundance of Proteins Involved in Stress Response and Cell Envelope Functions

The present study aims to elucidate the short-term proteomic response of this species to real microgravity during parabolic flight and suggests molecular rearrangements in the cell envelope of D. radiodurans could be linked with increased endogenous ROS production that contributes to the stress response.

CRISPRi in Deinococcus radiodurans

The programmable CRISPRi tool developed in this study will facilitate study of essential genes, hypothetical genes, cis-elements involved in radiation response as well as enable metabolic engineering in this organism and is amenable for implementing high-throughput approaches for such studies.

Conjugation-based genome engineering in Deinococcus radiodurans

The development of a simple and robust conjugation-based transformation system from E. coli to D. radiodurans allowing for the introduction of stable, replicating plasmids expressing antibiotic resistance markers and a protocol for creating sequential gene deletions in D. Radioduran by targeting re-striction-modification system genes.

Microbial Pathogenicity in Space

The current review intends to better clarify questions in order to facilitate future activities in space and assure the reduction of any possible health and environmental risks for the astronauts and for the locations being explored.

On the Problem of Optimal Microbiological Decontamination of Air Environment and Surfaces

Modern civilization, providing economic and social progress, at the same time objectively creates—sometimes close to ideal—conditions for the spread of various infections. The catastrophic

An Overview of Astrobiology and Microbial Survival in Space

With this review article, we aim to give a general overview on the environmental conditions in the different layers of space extending to above the Kármán line and also assessing the various

Database of space life investigations and bioinformatics of microbiology in extreme environments

The Space Life Investigation Database (SpaceLID), which collected SLIs from published academic papers, provides detailed menu search facilities and categorized contents about the studied phenomena, materials, experimental procedures, analysis methods, and research outcomes of 448 SLIs, including 232 SLIs not covered by the established databases.



Survival of Deinococcus geothermalis in Biofilms under Desiccation and Simulated Space and Martian Conditions.

A substantial proportion of the D. geothermalis population remained viable under all stress conditions tested, and in most cases the biofilm form proved advantageous for surviving space and Mars-like conditions.

Resistance of bacterial endospores to outer space for planetary protection purposes--experiment PROTECT of the EXPOSE-E mission.

The data demonstrate the high chance of survival of spores on a Mars mission, if protected against solar irradiation, and will have implications for planetary protection considerations.

Microorganisms and biomolecules in space environment experiment ES 029 on Spacelab-1.

  • G. HorneckH. Bucker P. Weber
  • Physics
    Advances in space research : the official journal of the Committee on Space Research
  • 1984

Survival of rock-colonizing organisms after 1.5 years in outer space.

Evidence is provided of the differential hardiness of cryptoendolithic communities in space, which some-but not all-of those most robust microbial communities from extremely hostile regions on Earth are also partially resistant to the even more hostile environment of outer space.

Protection of Bacterial Spores in Space, a Contribution to the Discussion on Panspermia

The data suggest that in a scenario of interplanetary transfer of life, small rock ejecta of a few cm in diameter could be sufficiently large to protect bacterial spores against the intense insolation; however, micron-sized grains, as originally requested by Panspermia, may not provide sufficient protection for spores to survive.

Responses ofBacillus subtilis spores to space environment: Results from experiments in space

  • G. Horneck
  • Physics
    Origins of life and evolution of the biosphere
  • 2005
Onboard of several spacecrafts, spores of Bacillus subtilis were exposed to selected parameters of space, such as space vacuum, different spectral ranges of solar UV-radiation and cosmic rays, applied separately or in combination, and their survival and genetic changes after retrieval are studied.

The Possible Interplanetary Transfer of Microbes: Assessing the Viability of Deinococcus spp. Under the ISS Environmental Conditions for Performing Exposure Experiments of Microbes in the Tanpopo Mission

It is concluded that aggregated deinococcal cells will survive the yearlong exposure experiments of the International Space Station, and it is proposed that microbial cells can aggregate as an ark for the interplanetary transfer of microbes, and is named ‘massapanspermia’.

Space Microbiology

The data support the likelihood of interplanetary transfer of microorganisms within meteorites, the so-called lithopanspermia hypothesis.