DNA DAMAGE BINDING PROTEIN 2 (DDB2) Shapes the DNA Methylation Landscape.

Abstract

In eukaryotes, DNA repair pathways help to maintain genome integrity and epigenomic patterns. However, the factors at the nexus of DNA repair and chromatin modification/remodeling remain poorly characterized. Here, we uncover a previously unrecognized interplay between the DNA repair factor DNA DAMAGE-BINDING PROTEIN 2 (DDB2) and the DNA methylation machinery in Arabidopsis thaliana. Loss-of-function mutation in DDB2 leads to genome-wide DNA methylation alterations. Genetic and biochemical evidence indicate that at many repeat loci, DDB2 influences de novo DNA methylation by interacting with ARGONAUTE 4 (AGO4) and by controlling the local abundance of 24-nt short interfering RNAs (siRNAs). We also show that DDB2 regulates active DNA demethylation mediated by REPRESSOR OF SILENCING 1 (ROS1) and DEMETER LIKE-3 (DML3). Together, these findings reveal a role for the DNA repair factor DDB2 in shaping the A. thaliana DNA methylation landscape in the absence of applied genotoxic stress.

DOI: 10.1105/tpc.16.00474

Cite this paper

@article{Schalk2016DNADB, title={DNA DAMAGE BINDING PROTEIN 2 (DDB2) Shapes the DNA Methylation Landscape.}, author={Catherine Schalk and Stephanie Drevensek and Amira Kramdi and Mohamed Kassam and Ikhlak Ahmed and Valerie Cognat and Stefanie Graindorge and Marc Bergdoll and Nicolas Baumberger and Dimitri Heintz and Chris Bowler and Pascal Genschik and Fredy Barneche and Vincent Colot and Jean Molinier}, journal={The Plant cell}, year={2016} }