DNA Binding of the Glucocorticoid Receptor Is Not Essential for Survival

@article{Reichardt1998DNABO,
  title={DNA Binding of the Glucocorticoid Receptor Is Not Essential for Survival},
  author={Holger M Reichardt and Klaus H. Kaestner and Jan P Tuckermann and Oliver Kretz and Oliver Wessely and Rudolf Bock and Peter Gass and Wolfgang Schmid and Peter Herrlich and Peter Angel and Günther Schütz},
  journal={Cell},
  year={1998},
  volume={93},
  pages={531-541}
}
Transcriptional regulation by the glucocorticoid receptor (GR) is essential for survival. Since the GR can influence transcription both through DNA-binding-dependent and -independent mechanisms, we attempted to assess their relative importance in vivo. In order to separate these modes of action, we introduced the point mutation A458T into the GR by gene targeting using the Cre/loxP system. This mutation impairs dimerization and therefore GRE-dependent transactivation while functions that… Expand

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