DNA Base Identification by Electron Microscopy

  title={DNA Base Identification by Electron Microscopy},
  author={David C Bell and W Thomas and Katelyn Marie Murtagh and Cheryl A. Dionne and Adam C. Graham and Jobriah E Anderson and William Roy Glover},
  journal={Microscopy and Microanalysis},
  pages={1049 - 1053}
Abstract Advances in DNA sequencing, based on fluorescent microscopy, have transformed many areas of biological research. However, only relatively short molecules can be sequenced by these technologies. Dramatic improvements in genomic research will require accurate sequencing of long (>10,000 base-pairs), intact DNA molecules. Our approach directly visualizes the sequence of DNA molecules using electron microscopy. This report represents the first identification of DNA base pairs within intact… 

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Determination of base sequence in nucleic acids with the electron microscope: visibility of a marker.

  • M. BeerMoudrianakisen
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 1962
The results presented below indicate that three to six uranyl ions are coupled to each marker group during the staining procedure and that such marker groups give a detectable level of visibility.

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  • Chemistry
    Annual review of biophysics and bioengineering
  • 1979
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