DNA Barcode Efficacy for the Identification of Economically Important Scale Insects (Hemiptera: Coccoidea) in South Africa

  title={DNA Barcode Efficacy for the Identification of Economically Important Scale Insects (Hemiptera: Coccoidea) in South Africa},
  author={Mamadi Theresa Sethusa and Ian M. Millar and Kowiyou Yessoufou and Adriaana Jacobs and Michelle van der Bank and Herman van der Bank},
Scale insects (Sternorrhyncha: Coccoidea) are one of the most invasive groups of insects. They are sedentary, cryptic, and often resemble the plant parts that they feed on. This coupled with increased international trade in fresh agricultural produce, makes them a major quarantine risk. An important limitation in controlling these pests involves species identification. When scale insects are intercepted on imported produce, they must be rapidly and accurately identified, using morphology-based… 

DNA barcoding of common soft scales (Hemiptera: Coccoidea: Coccidae) in China

The robustness of DNA barcoding for species discrimination of soft scales with two molecular markers (COI and 28S) is demonstrated and provides a reliable barcode library and rapid diagnostic tool for common soft scales in China.

Genetic diversity of armored scales (Hemiptera: Diaspididae) and soft scales (Hemiptera: Coccidae) in Chile

Morphological and DNA data were congruent, except for three species (Aspidiotus nerii, Hemiberlesia rapax and Coccus hesperidum) in which DNA data revealed highly differentiated lineages.

DNA Barcoding of Mealybugs (Hemiptera: Coccoidea: Pseudococcidae) From Mainland China

This study corroborates the utility of the COI and 28S genes in the rapid identification of mealybugs, and the barcode library provided will create an effective identification system for mealybug pest management in China.

Molecular Species Delimitation and Morphology of Aquatic and Sub-Aquatic Bugs (Heteroptera) in Cameroon

A good congruence between species a priori identified based on morphology from adult morphotypes and molecular putative species is obtained and molecular identification has allowed the association of 86% of nymphs with adults.

Molecular identification of two morphologically similar Eulecanium species: E. giganteum and E. kuwanai (Hemiptera: Coccidae)

Mitochondrial cytochrome c oxidase I (COI) and the D2–D3 expansion segments of 28S rDNA were used for accurate identification of these two morphologically similar Eulecanium species from 19 different locations in China.

Re-evaluation of the discriminatory power of DNA barcoding on some specimens of African Cyprinidae (subfamilies Cyprininae and Danioninae)

The results indicated the existence of barcode gaps, a discriminatory power of COI ranging from 79 % to 92 %, and that most nodes form well-supported monophyletic clades on an NJ tree, and indicate the utility ofCOI-based phylogenies for a wide spectrum of ecological questions related to African Cyprinidae.

Genetic variability on worldwide populations of the scale insect Pulvinariella mesembryanthemi

A genetic analysis including native and exotic populations of the South African scale insect Pulvinariella mesembryanthemi found the sequenced coxA gene was similar to that of the Rickettsiaceae family from the α-Proteobacteria, and close to other insect endosymbionts.

Molecular phylogeny of the family Coccidae (Hemiptera, Coccomorpha), with a discussion of their waxy ovisacs

This work presents the first molecular phylogeny of the family Coccidae based on DNA fragments of a mitochondrial gene (COI), nuclear ribosomal RNA genes (18S and 28S), and elongation factor‐1α (EF‐1 α) and proposes a new classification of certain groups.

Solanum mealybug Phenacoccus solani Ferris, 1918: New in Germany!

The mealybug Phenacoccus solani, which is known from grapevines in North America and Asia, has overwintered outdoors on ornamental plants in the Palatinate wine growing region.

Non-destructive DNA extraction from aphids: the application in virus - vector studies of Banana bunchy top virus (BBTV)

This non-destructive DNA extraction method for aphids was optimised, allowing the specimens to remain intact for use as vouchers for further morphological studies, and can be used in an early detection management strategy.



DNA barcodes for two scale insect families, mealybugs (Hemiptera: Pseudococcidae) and armored scales (Hemiptera: Diaspididae)

The feasibility of developing a comprehensive barcode library for scale insects is established and it is indicated that its construction will both create an effective system for identifying scale insects and reveal taxonomic situations worthy of deeper analysis.

DNA barcoding of six Ceroplastes species (Hemiptera: Coccoidea: Coccidae) from China

Mitochondrial cytochrome c oxidase I (COI) sequences and the D2 expansion segments of the large subunit ribosomal RNA gene 28S were used for accurate identification of six Ceroplastes species from 20 different locations in China, indicating that the standard barcode region of COI can efficiently identify similar CeropLastes species.

Molecular Identification of Mealybugs (Hemiptera: Pseudococcidae) Found on Korean Pears

A polymerase chain reaction-based method for species identification was developed for six mealybug species known to infest Korean pears including two regulated insects including tworegulated insects, Planococcus kraunhiae (Kuwana) and Crisicoccus matsumotoi (Siraiwa).

Molecular and morphological characterisation of Pseudococcidae surveyed on crops and ornamental plants in Spain

This study characterised 33 mealybug populations infesting crops and ornamental plants in eastern Spain, using a combination of molecular and morphological techniques, which led to the identification of ten species and provides sequence data for three previously unsequenced species, contributing to the phylogenetic knowledge of the family Pseudococcidae.

DNA barcoding cannot reliably identify species of the blowfly genus Protocalliphora (Diptera: Calliphoridae)

Investigation of barcoding in a sample comprising 12 species of the blow fly genus Protocalliphora, known to be infected with the endosymbiotic bacteria Wolbachia, concludes that identification at the species level based on mitochondrial sequence might not be possible for many insects.

Ten species in one: DNA barcoding reveals cryptic species in the neotropical skipper butterfly Astraptes fulgerator.

The results add to the evidence that cryptic species are prevalent in tropical regions, a critical issue in efforts to document global species richness, and illustrate the value of DNA barcoding, especially when coupled with traditional taxonomic tools, in disclosing hidden diversity.

DNA markers to disentangle complexes of cryptic taxa in mealybugs (Hemiptera: Pseudococcidae)

The five markers studied successfully distinguished all species identified by morphological examination, disentangled complexes of species by revealing intraspecific genetic variation and identified a set of closely related taxa for which taxonomic status requires clarification through further studies, and facilitated the inference of phylogenetic relationships between the characterized taxa.

Identifying spiders through DNA barcodes

This study demonstrates that sequence diversity in a standard segment of the mitochondrial gene coding for cytochrome c oxidase I (COI) is highly effective in discriminating spider species and establishes the potential of COI as a rapid and accurate identification tool for biodiversity surveys of spiders.

Identification of mealybug pest species (Hemiptera: Pseudococcidae) in Egypt and France, using a DNA barcoding approach

Genetic variation between populations considered to belong to the same species is found, justifying further investigation of the possible occurrence of complexes of cryptic taxa.

Identification of the immature instars of mealybugs (Hemiptera: Pseudococcidae) found on citrus in Australia

An identification guide is provided to most of the immature stages of six mealybug species that might be collected from citrus (Rutaceae) in Australia for foreign quarantine inspections because mealybugs intercepted on Australian citrus exports are often nymphs.