DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.

@article{Kimoto2016DNAAG,
  title={DNA Aptamer Generation by Genetic Alphabet Expansion SELEX (ExSELEX) Using an Unnatural Base Pair System.},
  author={Michiko Kimoto and Ken-ichiro Matsunaga and Ichiro Hirao},
  journal={Methods in molecular biology},
  year={2016},
  volume={1380},
  pages={47-60}
}
Genetic alphabet expansion of DNA using unnatural base pair systems is expected to provide a wide variety of novel tools and methods. Recent rapid progress in this area has enabled the creation of several types of unnatural base pairs that function as a third base pair in polymerase reactions. Presently, a major topic is whether the genetic alphabet expansion system actually increases nucleic acid functionalities. We recently applied our unnatural base pair system to in vitro selection (SELEX… CONTINUE READING