DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo

@article{Zubradt2017DMSMaPseqFG,
  title={DMS-MaPseq for genome-wide or targeted RNA structure probing in vivo},
  author={Meghan M. Zubradt and Paromita D Gupta and Sitara Persad and Alan M. Lambowitz and Jonathan S. Weissman and Silvi Rouskin},
  journal={Nature Methods},
  year={2017},
  volume={14},
  pages={75-82}
}
Coupling of structure-specific in vivo chemical modification to next-generation sequencing is transforming RNA secondary structure studies in living cells. The dominant strategy for detecting in vivo chemical modifications uses reverse transcriptase truncation products, which introduce biases and necessitate population-average assessments of RNA structure. Here we present dimethyl sulfate (DMS) mutational profiling with sequencing (DMS-MaPseq), which encodes DMS modifications as mismatches… CONTINUE READING
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Genome-wide DMS-MaPseq for in vivo RNA structure determination

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Target-specific DMS-MaPseq for in vivo RNA structure determination

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