DMI-1, a new DNA methyltransferase inhibitor produced by Streptomyces sp. strain No. 560.

  title={DMI-1, a new DNA methyltransferase inhibitor produced by Streptomyces sp. strain No. 560.},
  author={K. Suzuki and K. Nagao and J. Tokunaga and M. Hirosawa and H. Tsubone and M. Uyeda},
  journal={Journal of enzyme inhibition},
  volume={9 4},
A new inhibitor of DNA methyltransferase named DMI-1 has been discovered in the culture filtrate of Streptomyces sp. strain No. 560. DMI-1 was purified by extraction with ethyl acetate followed by Diaion HP-20SS and silica gel column chromatography. The structure of DMI-1 was determined to be 8-methylpentadecanoic acid (C16H32O2). DMI-1 is a novel inhibitor of methyltransferase isolated from microorganisms and is structurally different from sinefungin and A9145C which are structural analogs of… Expand
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Inhibition of DNA methyltransferase by microbial inhibitors and fatty acids.
The results suggest that the length of the carbon chain, the number of double bonds and the presence of a carboxyl group and branched methyl group in the fatty acid molecule may play an important role in the inhibition of DNA methyltransferase. Expand
DMI-2 and DMI-3, DNA methyltransferase inhibitors produced by Streptomyces sp. strain no. 560.
DMI-2 and DMI-3 showed strong inhibition against N6-methyladenine-DNA methyltransferase (M. Eco RI) and Eco RI in a competitive manner with respect to plasmid pUC19 and SAM. Expand
1513-DMIa and 1513-DMIb, DNA methyltransferase inhibitors produced by Streptomyces sp. strain No. 1513.
Two new methyltransferase inhibitors were isolated from the culture filtrate of Streptomyces sp. strain No. 1513 and named 1513-DMIa and 1513-DMIb. 1513-DMIa and 1513-DMIb were distinguished inExpand
Topostatin, a novel inhibitor of topoisomerases I and II produced by Thermomonospora alba strain No. 1520. I. Taxonomy, fermentation, isolation and biological activities.
A novel inhibitor of topoisomerases designated as topostatin was isolated from the culture filtrate of Thermomonospora alba strain No. 1520, but did not exhibit any antimicrobial activity against Gram-positive and Gram-negative bacteria, yeasts and fungi. Expand
[Metabolites produced by actinomycetes--antiviral antibiotics and enzyme inhibitors].
  • M. Uyeda
  • Chemistry, Medicine
  • Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan
  • 2004
Using bacitracin-resistant strains, enhanced and astringent production of fattiviracin was achieved, and FV-13, which has the longest fatty acid chains in the molecule, was dramatically enhanced by a C(55)-isoprenyl phosphate metabolism. Expand
5-Methylcytosine as a marker for the monitoring of DNA methylation.
  • J. Havliš, M. Trbušek
  • Chemistry, Medicine
  • Journal of chromatography. B, Analytical technologies in the biomedical and life sciences
  • 2002
The currently employed techniques are reviewed both from the point of view of their relevance in genomic research and of their analytical application. Expand


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