DIDS (4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid) increases iodide trapping, inhibits thyroperoxidase and antagonizes the TSH-induced apical iodide efflux in porcine thyroid cells

@article{AmphouxFazekas1998DIDSA,
  title={DIDS (4,4′-diisothiocyanatostilbene-2,2′-disulfonic acid) increases iodide trapping, inhibits thyroperoxidase and antagonizes the TSH-induced apical iodide efflux in porcine thyroid cells},
  author={T. Amphoux-Fazekas and Nezha Samih and Silva Hovsepian and A. Aouani and G. Fayet},
  journal={Molecular and Cellular Endocrinology},
  year={1998},
  volume={141},
  pages={129-140}
}
4,4'-Di-isothiocyanatostilbene-2,2'-disulfonic acid (DIDS), an inhibitor of several anionic channels and transporters including the band 3 protein of the red blood cell membrane was tested on iodide metabolism in cultured porcine thyroid cells. We used three experimental cell culture models: (i) forskolin-stimulated correctly inside-in polarized follicle-associated thyroid cells cultured onto plastic support (ii) suspensions of isolated cells derived from such cultures (iii) polarized… CONTINUE READING