DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population

  title={DHPLC technology for high-throughput detection of mutations in a durum wheat TILLING population},
  author={Pasqualina Colasuonno and Ornella Incerti and Maria Luisa Lozito and Rosanna Simeone and Agata Gadaleta and Antonio Blanco},
  journal={BMC Genetics},
Durum wheat (Triticum turgidum L.) is a cereal crop widely grown in the Mediterranean regions; the amber grain is mainly used for the production of pasta, couscous and typical breads. Single nucleotide polymorphism (SNP) detection technologies and high-throughput mutation induction represent a new challenge in wheat breeding to identify allelic variation in large populations. The TILLING strategy makes use of traditional chemical mutagenesis followed by screening for single base mismatches to… CONTINUE READING
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