DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA

@article{Naorem2017DGRMT,
  title={DGR mutagenic transposition occurs via hypermutagenic reverse transcription primed by nicked template RNA},
  author={Santa S. Naorem and Jin Han and Shufang Wang and William R. Lee and X. Heng and Jeff F. Miller and H. Guo},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2017},
  volume={114},
  pages={E10187 - E10195}
}
  • Santa S. Naorem, Jin Han, +4 authors H. Guo
  • Published 2017
  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
Significance Diversity-generating retroelements (DGRs) are in vivo sequence diversification machines that are widely distributed in bacteria, archaea, and their viruses. DGRs use a reverse transcriptase (RT)-mediated mechanism to diversify protein-encoding genes to facilitate adaptation of their hosts to changing environments. Here, we demonstrate that the Bordetella phage DGR-encoded RT uses the 3′-OH of a nicked template RNA to initiate reverse transcription, during which random nucleotides… Expand
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