DGGE and real-time PCR analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce.

@article{Zwielehner2008DGGEAR,
  title={DGGE and real-time PCR analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce.},
  author={Jutta Zwielehner and Michael Handschur and Astrid Michaelsen and Selen Irez and Michael Demel and Ewald B. M. Denner and Alexander G Haslberger},
  journal={Molecular nutrition & food research},
  year={2008},
  volume={52 5},
  pages={614-23}
}
Food associated indigenous microbial communities exert antagonistic effects on pathogens and may routinely deliver health relevant microorganisms to the GI tract. By using molecular, culture independent methods including PCR-DGGE of 16S rDNA-coding regions and real-time PCR (RT-PCR) as well as BIOLOG metabolic fingerprinting, microbial communities on lettuce were analyzed in samples from fields, from supermarkets and soil. Amplified 16S rRNA gene sequences (57.7%) could be assigned to species… CONTINUE READING