DEVELOPMENTAL BIOLOGY 68,533-545(979) Localized Ultraviolet Laser Microbeam Irradiation of Early Drosophila Embryos: Fate Maps Based on Location and Frequency of Adult Defects


Drosophila embryos were locally irradiated with a 257-nm laser mierobeam during blastoderm and germ band stages. Depending on stage and beam diameter (10-30 pm), from 0 to 45 nuclei were exposed to the uv radiation. The doses used, 5 or 10 erg, did not eliminate nuclei or cells at onee, but up to 50% of the adult survivors from irradiated eggs carried… (More)


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