DEADSouth protein localizes to germ plasm and is required for the development of primordial germ cells in Xenopus laevis

  title={DEADSouth protein localizes to germ plasm and is required for the development of primordial germ cells in Xenopus laevis},
  author={T. Yamaguchi and Ayaka Taguchi and K. Watanabe and H. Orii},
  journal={Biology Open},
  pages={191 - 199}
  • T. Yamaguchi, Ayaka Taguchi, +1 author H. Orii
  • Published 2012
  • Biology, Medicine
  • Biology Open
  • Summary DEADSouth mRNA is a component of germ plasm in Xenopus laevis and encodes a DDX25 DEAD-box RNA helicase. To determine the intracellular localization of DEADSouth protein, we injected mRNA encoding DEADSouth tagged with mCherry fluorescent protein into fertilized eggs from transgenic Xenopus expressing EGFP fused with a mitochondrial targeting signal. The DEADSouth-mCherry fusion protein was localized to the germ plasm, a mitochondria-rich region in primordial germ cells (PGCs… CONTINUE READING
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