DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease

Abstract

A method to quantify resting spores of Olpidium virulentus, fungal vector of Mirafiori lettuce big-vein virus, which causes lettuce big-vein disease, in lettuce roots was developed using a DAS-ELISA with a polyclonal antibody against the resting spores and used to evaluate the relationship between the resting spore density in soil and disease severity. When lettuce seedlings were grown on soils containing a powder made from diseased roots with a known number of resting spores, symptoms developed from ca. 102 spores/g of soil or more. This simple method can be used in soil diagnosis and risk assessment of the disease.

DOI: 10.1007/s10327-015-0586-y

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Cite this paper

@article{Nomiyama2015DASELISAQO, title={DAS-ELISA quantification of resting spores of Olpidium virulentus in roots and correlation between resting spore density in soil and severity of lettuce big-vein disease}, author={Koji Nomiyama and Takahide Sasaya and Hiroyuki Sekiguchi and Keisuke Tomioka and Hideki Osaki and Minoru Takeshita and Toshiaki Takehara and Naruto Furuya and Kenichi Tsuchiya}, journal={Journal of General Plant Pathology}, year={2015}, volume={81}, pages={243-248} }