DARFA: a novel technique for studying differential gene expression and bacterial comparative genomics.

@article{Kim2005DARFAAN,
  title={DARFA: a novel technique for studying differential gene expression and bacterial comparative genomics.},
  author={Seonhoe Kim and Soyeon Park and Sorim Choung and Han-Oh Park and Young Chul Choi},
  journal={Biochemical and biophysical research communications},
  year={2005},
  volume={336 1},
  pages={168-74}
}
We have developed a powerful method, named differential analysis of restriction fragments amplification (DARFA), which enables researchers to perform comprehensive transcriptome analysis as well as bacterial DNA fingerprinting. The key feature of this novel technique lies within the usage of a type IIS enzyme, Hpy188III, which cleaves cDNA or genomic DNA at a TC/NNGA recognition sequence. Cleavage at this particular site results in the production of a pool of restriction fragments which can be… CONTINUE READING
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