DAFS: a data-adaptive flag method for RNA-sequencing data to differentiate genes with low and high expression

Next-generation sequencing (NGS) has advanced the application of high-throughput sequencing technologies in genetic and genomic variation analysis. Due to the large dynamic range of expression levels, RNA-seq is more prone to detect transcripts with low expression. It is clear that genes with no mapped reads are not expressed; however, there is ongoing… CONTINUE READING