• Medicine
  • Published in
    Clinical and experimental…
    2002

D-penicillamine cooperates with copper sulfate to enhance the surface expression of functional Fas antigen in rheumatoid synovial fibroblasts via the generation of hydrogen peroxide.

@article{Harada2002DpenicillamineCW,
  title={D-penicillamine cooperates with copper sulfate to enhance the surface expression of functional Fas antigen in rheumatoid synovial fibroblasts via the generation of hydrogen peroxide.},
  author={Shoji Harada and Eiji Sugiyama and Hideki Taki and Kenta Shinoda and Toshiro Fujita and Muneharu Maruyama and Masashi Kobayashi},
  journal={Clinical and experimental rheumatology},
  year={2002},
  volume={20 4},
  pages={
          469-76
        }
}
OBJECTIVE D-penicillamine (DP) has been shown to cooperate with copper ion to inhibit cell growth in a variety of cell types. To determine whether this inhibitory action is involved in Fas-mediated apoptosis, we examined the effect of DP and copper sulfate on the expression and function of Fas antigen in rheumatoid synovial fibroblasts (RSFs). METHODS The expression of Fas antigen on the cell surface was determined by flow cytometric analysis. Western blot analysis was performed to examine… CONTINUE READING