D-glucose: preferential binding to brush borders disrupted with tris(hydroxymethyl)aminomethane.

@article{Faust1967DglucosePB,
  title={D-glucose: preferential binding to brush borders disrupted with tris(hydroxymethyl)aminomethane.},
  author={Robert G. Faust and S L Wu and M L Faggard},
  journal={Science},
  year={1967},
  volume={155 3767},
  pages={1261-3}
}
The actively transported sugar D-glucose binds to brush borders disrupted with tris(hydroxymethyl)aminomethane in preference to D-mannose and L-glucose, which are not actively transported. This preferential binding of D-glucose is not dependent on either added Na(+) or adenosine triphosphatase activity stimulated by Na(+) with K(+) and Mg(2+), but it is temperature-dependent and is completely inhibited by 0.1 millimolar phlorizin and 1 millimolar mercuric chloride.