D-glucosamine-induced changes in nucleotide metabolism and growth of colon-carcinoma cells in culture.

  title={D-glucosamine-induced changes in nucleotide metabolism and growth of colon-carcinoma cells in culture.},
  author={E. Krug and Alain Zweibaum and C Schulz-Holstege and Dietrich Keppler},
  journal={The Biochemical journal},
  volume={217 3},
Human colon-carcinoma cells were exposed to D-glucosamine at 2.5, 5 and 10 mM, concentrations that were growth-inhibitory but not cytocidal in the presence of a physiological glucose concentration. Labelling of these HT-29 cells with D-[14C]-glucosamine, followed by nucleotide analyses, demonstrated that UDP-N-acetyl-hexosamines represented the major intracellular nucleotide pool and the predominant metabolite of the amino sugar. D-[14C]Glucosamine was not a precursor of UDP-glucosamine. After… Expand
The relationship between intracellular UDP-N-acetyl hexosamine nucleotide pool and monoclonal antibody production in a mouse hybridoma.
It is concluded that UDP-N-acetyl hexosamine does not act as a mediator of enhanced rates of monoclonal antibody synthesis in the hybridoma cell culture system. Expand
Uridine enhances the cytotoxic effect of D-glucosamine in rat C6 glioma cells.
The findings suggest a connection between the accumulation of UDP-N-acetylhexosamines in the cells and the appearance of D-glucosamine cytotoxicity. Expand
UDP-N-acetylhexosamine modulation by glucosamine and uridine in NCI N-417 variant small cell lung cancer cells: 31P nuclear magnetic resonance results.
DPDE levels in N-417 cells retain the capacity to rapidly convert uridine to UTP despite low ATP and phosphocreatine levels, indicating expansion of the uridine pool may represent an additional metabolic reserve not yet addressed in the design of therapy options. Expand
D-glucosamine inhibits proliferation of human cancer cells through inhibition of p70S6K.
It is suggested that D-glucosamine can inhibit growth of cancer cells through dephosphorylation of p70S6K, an important signaling molecule involved in protein translation. Expand
UDP-jY-Acetylhexosamine Modulation by Glucosamine and Uridine in NCI N-417 Variant Small Cell Lung Cancer Cells: 31P Nuclear Magnetic Resonance Results1
Small cell lung cancer (SCLC) occurs as two neuroendocrine subtypes, SCLC-C (classic) and SCLC-V (variant). One reported difference is elevated levels of diphosphodiesters (DPDE) in the moreExpand
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Results indicate a possible role for UDP-amino sugars in the depression of ganglioside biosynthesis observed in vivo after GalN administration. Expand
Biochemistry of growth inhibition by ammonium ions in mammalian cells
The intracellular amount of UDP‐GNAc correlates with the level of growth inhibition in mammalian cell lines and has been shown to increase with an elevated cultivation pH and to be independent of the inhibition of transcription and translation processes. Expand
Role of Nucleotide Sugar Pools in the Inhibition of NCAM Polysialylation by Ammonia
In an attempt to increase NCAM polysialylation, N‐acetylmannosamine and cytidine were added to cultures in order to circumvent the feedback inhibition of CMP‐sialic acid synthesis, but this only slightly increased PolySia levels and failed to counter ammonia's inhibition of NCAMPolySia. Expand
Uridylate-trapping sugar analogs in combination with inhibitors of uridylate synthesis de novo and 5-fluorouridine.
Determination of the rates of de novo pyrimidine synthesis, of the formation of RNA pyrimidines, and of pyridine nucleoside excretion indicates that de noVO synthesis provides only about 67% of the pyrimids required for the consuming processes. Expand
Uridine diphospho sugars and related hexose phosphates in the liver of hexosamine-treated rats: identification using 31P-[1H] two-dimensional NMR with HOHAHA relay.
UDP-GlcN and UDP-GalN were not commercially available, but their presence was established in the extracts after GalN treatment by obtaining relay spectra for a mixture of the compounds produced in situ enzymatically, without purification. Expand