D-Tagatose manufacture through bio-oxidation of galactitol derived from waste xylose mother liquor

  title={D-Tagatose manufacture through bio-oxidation of galactitol derived from waste xylose mother liquor},
  author={Sha Feng and Yucong Zheng and Jiao Chen and Kequan Chen and Fei Cao and Ming Yan and Pingkai Ouyang},
  journal={Green Chemistry},
In this study, the bio-oxidation of galactitol to the valuable D-tagatose is presented. This proposed strategy could start with the refined by-product of waste xylose mother liquor formed by chemical hydrogenation. Through a computationally guided enzyme screening approach, a robust polyol dehydrogenase (PdPDH) was rapidly identified from a massive number of candidates. When coupled with a water-forming NADH oxidase (StNOX) for environmentally benign cofactor regeneration, subsequent reaction… 

Elegant and Efficient Biotransformation for Dual Production of d-Tagatose and Bioethanol from Cheese Whey Powder.

This bioprocess not only provides an efficient method for the functionalization of byproduct whey, but also offsets the high production cost of d-tagatose and bioethanol.

A Three-Step Process for the Bioconversion of Whey Permeate into a Glucose-Free D-Tagatose Syrup

A sustainable three-stage process for the revaluation of cheese whey permeate into D-tagatose, a rare sugar with functional properties used as sweetener, yielding a glucose-free syrup containing D- tagatose and galactose in a ratio 1:2 (w/w).

Design of a self-sufficient hydride-shuttling cascade for concurrent bioproduction of 7,12-dioxolithocholate and l-tert-leucine

A highly atom-economic self-sufficient hydride-shuttling cascade to concurrently obtain two pharmaceutically important building blocks in which oxidation of cholic acid and reductive amination of trimethylpyruvic acid were integrated for redox self-recycling.

Efficient biocatalytic synthesis of D-tagatose 1,6-diphosphate by LacC-catalysed phosphorylation of D-tagatose 6-phosphate

An efficient biocatalytic synthesis has been developed for the phosphorylation of D-tagatose 1,6-diphosphate at its C1-position by using the recombinant D- tagatose 6-phosphate kinase and the phosphoenolpyruvate/pyruve kinase-system for ATP regeneration.

Similarity based enzymatic retrosynthesis

The development and demonstration of the single-step enzymatic retrosynthesis strategy using natural transformations, provides a first step towards solving the challenging problem of incorporating both enzyme- and organic-chemistry based transformations into a computer aided synthesis planning workflow.

Biocatalytic Oxidation of Alcohols

The current state-of-the-artenzymatic methods for the oxidation of alcohols are outlined and current limitations and promising solutions are discussed.

Production of D-tagatose: A review with emphasis on subcritical fluid treatment

In this review, all potential methods for producing tagatose were summarized with the recent publications and a novel method, subcritical fluid treatment was also introduced and discussed for its potential.

Horse Liver Alcohol Dehydrogenase-Catalyzed Oxidative Lactamization of Amino Alcohols

A direct synthesis of lactams (5, 6, and 7-membered) starting from amino-alcohols in a bienzymatic cascade is reported, representing a direct approach for biocatalytic oxidative lactamization reaction.



Tagatose: properties, applications, and biotechnological processes

  • D. Oh
  • Biology, Engineering
    Applied Microbiology and Biotechnology
  • 2007
The characterization of many l-arabinose isomerases and their d- Tagatose production is compared and protein engineering and immobilization of the enzyme for increasing the conversion rate of d-galactose to d-tagatose are reviewed.

Xylitol production from waste xylose mother liquor containing miscellaneous sugars and inhibitors: one-pot biotransformation by Candida tropicalis and recombinant Bacillus subtilis

The results demonstrate that the one-pot procedure is a viable option for the industrial application of WXML to produce value-added chemicals and provides useful information on the combination of biotechnological processes for the biotransformation of other compounds.

L-Arabinose isomerase and its use for biotechnological production of rare sugars

The crystal structure of AI has been disclosed, which sheds light on the understanding of AI structure and catalytic mechanism at molecular levels, and the structural biology study of AI is initiated.

Current studies on biological tagatose production using l-arabinose isomerase: a review and future perspective

  • P. Kim
  • Biology
    Applied Microbiology and Biotechnology
  • 2004
The properties and metabolism of tagatose, a hexoketose monosaccharide sweetener, as well as its commercial importance are described and potential solutions are suggested.

A Feasible Enzymatic Process for d‐Tagatose Production by an Immobilized Thermostable l‐Arabinose Isomerase in a Packed‐Bed Bioreactor

To develop a feasible enzymatic process for d‐tagatose production, a thermostable l‐arabinose isomerase, Gali152, was immobilized in alginate, and the galactose isomerization reaction conditions were

Bioconversion of d-galactose to d-tagatose: continuous packed bed reaction with an immobilized thermostable l-arabinose isomerase and efficient purification by selective microbial degradation

The established production and separation methods further potentiate the industrial production of d-tagatose via bioconversion and biopurification processes and a novel method for d- tagatose purification were studied.

Efficient Production of l-Ribose with a Recombinant Escherichia coli Biocatalyst

A new synthetic platform with potential for the production of several rare sugars, with l-ribose as the model target, is described, and the synthetic utility was demonstrated for production of l- ribose and the system was improved using shaken flask experiments.