D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants.

@article{Wienstroer2012DLactateDA,
  title={D-Lactate dehydrogenase as a marker gene allows positive selection of transgenic plants.},
  author={Judith Wienstroer and Martin K M Engqvist and Hans-Henning Kunz and U. -I. Fl{\"u}gge and Veronica G Maurino},
  journal={FEBS letters},
  year={2012},
  volume={586 1},
  pages={36-40}
}
D-Lactate negatively affects Arabidopsis thaliana seedling development in a concentration-dependent manner. At media D-lactate concentrations greater than 5-10mM the development of wild-type plants is arrested shortly after germination whereas plants overexpressing the endogenous D-lactate dehydrogenase (D-LDH) detoxify D-lactate to pyruvate and survive. When the transgenic plants are further transferred to normal growth conditions they develop indistinguishably from the wild type. Thus, D-LDH… CONTINUE READING

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