D-(-)-beta-hydroxybutyrate-induced effects on mouse embryos in vitro.

  title={D-(-)-beta-hydroxybutyrate-induced effects on mouse embryos in vitro.},
  author={Edward Sidney Hunter and Thomas W. Sadler},
  volume={36 2},
The effects of the "physiologically occurring" D-isomer of beta-hydroxybutyrate (D-BOHB) were evaluated in neurulating mouse embryos by using the technique of whole embryo culture. Following a 24-hour culture period D-BOHB induced malformations and growth retardation in a concentration-dependent manner. At a 48 mM concentration essentially all embryos exhibited neural tube defects, and decreased rates of glucose metabolism by the pentose phosphate pathway (PPP) and Krebs cycle were observed… CONTINUE READING