Cytotoxicity of ascorbate and other reducing agents towards cultured fibroblasts as a result of hydrogen peroxide formation

@article{Peterkofsky1977CytotoxicityOA,
  title={Cytotoxicity of ascorbate and other reducing agents towards cultured fibroblasts as a result of hydrogen peroxide formation},
  author={B. Peterkofsky and W. Prather},
  journal={Journal of Cellular Physiology},
  year={1977},
  volume={90}
}
  • B. Peterkofsky, W. Prather
  • Published 1977
  • Biology, Medicine
  • Journal of Cellular Physiology
  • Several types of cultured fibroblasts, including chick embryo, human and mouse, were killed by the addition of sodium ascorbate at final concentrations of 0.05–0.25 mM to cultures at the time of inoculation or to attached cells. Ascorbate did not affect the attachment of cells to the substratum. The effect on chick embryo fibroblasts was visible by fours hours and by six hours almost all cells had swelled and were becoming detached. By 24 hours detached cells had either lysed or become crenated… CONTINUE READING
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