• Corpus ID: 209505950

Cytotoxicity evaluation of newly developed bi-functional, oligomer-based sealers and a methacrylate resin-based root canal sealer

@inproceedings{Banomyong2016CytotoxicityEO,
  title={Cytotoxicity evaluation of newly developed bi-functional, oligomer-based sealers and a methacrylate resin-based root canal sealer},
  author={Danuchit Banomyong and Lalida Ongchavalit and Kallaya Yanpiset},
  year={2016}
}

• D. Banomyong, Lalida Ongchavalit, K. Yanpiset
• Published 2016
• Materials Science

Materials and methods: L929 cells were sub-cultured into 96-well plates with 100 μl (1 x 10 cel ls) per well. Four formulas of the oligomer-based sealer (namely F68, F71, F72 and F75) and RealSeal SE sealer were prepared. Sealer rod (0.2 g) was produced in a polyethylene tube and set in a self-curing mode at room temperature for 7 days. Elute of a sealer rod was prepared and diluted with MEM medium at 1 (no dilution), 10, 100 and 1,000 times. Next, 100 μl of elute was added into a well, 16 wells of each dilution. MEM medium at 100 μl, without any elute, was used as a blank control. After 24-h incubation, MTT solution at 50 μl was added into each well and then incubated for 2 h. Optical density was read at 570-nm wavelength, and cell viability was determined as a percentage of the blank control value.