Corpus ID: 209505950

Cytotoxicity evaluation of newly developed bi-functional, oligomer-based sealers and a methacrylate resin-based root canal sealer

  title={Cytotoxicity evaluation of newly developed bi-functional, oligomer-based sealers and a methacrylate resin-based root canal sealer},
  author={D. Banomyong and Lalida Ongchavalit and K. Yanpiset},
Materials and methods: L929 cells were sub-cultured into 96-well plates with 100 μl (1 x 10 cel ls) per well. Four formulas of the oligomer-based sealer (namely F68, F71, F72 and F75) and RealSeal SE sealer were prepared. Sealer rod (0.2 g) was produced in a polyethylene tube and set in a self-curing mode at room temperature for 7 days. Elute of a sealer rod was prepared and diluted with MEM medium at 1 (no dilution), 10, 100 and 1,000 times. Next, 100 μl of elute was added into a well, 16… Expand

Figures from this paper

In vitro antibacterial activity of oligomer-based and calcium silicate-based root canal sealers
Objective: To evaluate antibacterial effects of root canal sealers, oligomer-based (OB) and calcium silicate (BC) in comparison to epoxy-resin (AH Plus) and zinc oxide-eugenol (ZOE), againstExpand


Effect of time of extraction on the biocompatibility of endodontic sealers with primary human fibroblasts.
Evaluating the effects of different times of extraction on the cytotoxicity of six representatives of different root canal sealer groups with human gingival fibroblasts indicated that GuttaFlow was the least cytotoxic sealer tested. Expand
Multilayer and monolayer cell cultures in a cytotoxicity assay of root canal sealers.
There was no statistical difference in the percentage viability for each sealer in both the multilayer and the monolayer cell culture, and Apexit and AH-26 were less toxic than MU (Mahidol University) sealer, ROCANAL 3, RocANAL 2, and Endomethasone which demonstrated the same toxicity. Expand
Short-term cytotoxicity assessment of components of the epiphany resin-percha obturating system by indirect and direct contact millipore filter assays.
Cytotoxicity of set Epiphany sealer and Resilon was comparable with that of set AH-Plus and gutta-percha, and did not exceed that of freshly mixedAH-Plus. Expand
Cytotoxicity testing of endodontic sealers: a new method.
The results show that the ISO standards may strongly over-evaluate the cytotoxicity of the endodontic sealers, emphasize the difference among the sealer, and may clinically correspond to a large overfilling. Expand
Susceptibility of a polycaprolactone-based root canal-filling material to degradation. III. Turbidimetric evaluation of enzymatic hydrolysis.
The susceptibility of Resilon to biotic biodegradation by two hydrolases, lipase PS and cholesterol esterase, was investigated with a turbidimetric approach by measuring the optical density reductions in aqueous emulsions containing dissolved, filtered, surfactant-stabilized polymeric components of Resilus. Expand
Susceptibility of a polycaprolactone-based root canal filling material to degradation. II. Gravimetric evaluation of enzymatic hydrolysis.
Biodegradation of Resilon by bacterial and salivary enzymes warrants further investigation of their activities using cultures of endodontically relevant microbes and human saliva extracts. Expand
In vitro evaluation of bond strength and sealing ability of a new low-shrinkage, methacrylate resin-based root canal sealer.
The newly developed LSRCS, although not superior to AH Plus in bond strength or sealing ability, possesses monoblock potential and application prospects. Expand
Periapical reactions to calcium hydroxide-containing sealers and AH 26 in monkeys.
This sealer has not yet been tested in infected cases and its ability to be resorbed, which is an advantage when it precedes replacement by calcified tissue, may become a disadvantage if it is not followed by apical obliteration. Expand
Cytotoxicity of Epiphany and Resilon with a root model.
The cytotoxicity of Resilon +Epiphany, due mainly to Epiphany, decreased after 2 days to reach a level comparable with commonly used root canal sealers. Expand
Biocompatibility of furcal perforation repair material using cell culture technique: Ketac Molar versus ProRoot MTA.
Although Ketac Molar has the advantage of adhering to dentine, it is more cytotoxic to the PDL cells than MTA, and it is recommended not only to consider the sealing ability of the material with dentine but also the biocompatibility of material to the underlying tissue. Expand