Cytotoxicity assays using cryopreserved target cells pre-labeled with the fluorescent marker europium.

@article{Maley1990CytotoxicityAU,
  title={Cytotoxicity assays using cryopreserved target cells pre-labeled with the fluorescent marker europium.},
  author={Desmond Maley and Pierre Simon},
  journal={Journal of immunological methods},
  year={1990},
  volume={134 1},
  pages={
          61-70
        }
}
We describe a method for standardizing cytotoxicity assays by the use of cryopreserved, fluorescently labeled target cells. The cells are labeled in batches with the fluorogenic element europium (Eu) and frozen in multiple aliquots. Replicate aliquots can be thawed on different days and used for cytotoxicity assays. Thawed pre-labeled cells from the same batch are killed reproducibly, allowing cytotoxicity assays to be standardized. The target cells need only be thawed, washed, counted for… CONTINUE READING

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