Cytotoxicity and genotoxicity of chlorhexidine on macrophages in vitro.

  title={Cytotoxicity and genotoxicity of chlorhexidine on macrophages in vitro.},
  author={Yi-ching Li and Yu-hsiang Kuan and Shiuan-Shinn Lee and F M Huang and Yu-Chao Chang},
  journal={Environmental toxicology},
  volume={29 4},
Chlorhexidine (CHX) is the most widely used antiseptic for wound, skin disinfection, and dental hygiene. The aim of this study is to investigate the possible correlation between CHX-induced cytogenotoxicity and alterations in normal cell cycle on RAW264.7 macrophages. The cytotoxicity, mechanism of cell death, mitotic activity, and reactive oxygen species (ROS) generation were determined by tetrazolium bromide reduction assay, flow cytometry, cytokinesis-block proliferation index, and… CONTINUE READING