• DOI:10.1080/03601234.2017.1410385
• Corpus ID: 3287306

Cytotoxic activity of the bioactive principles from Ficus burtt-davyi

@article{Ogunlaja2018CytotoxicAO,
  title={Cytotoxic activity of the bioactive principles from Ficus burtt-davyi},
  author={Olumuyiwa O. Ogunlaja and Roshila Moodley and Moganavelli Singh and H. Baijnath and Sreekantha Babu Jonnalagadda},
  journal={Journal of Environmental Science and Health, Part B},
  year={2018},
  volume={53},
  pages={261 - 275}
}

• Olumuyiwa O. Ogunlaja, R. Moodley, +2 authors S. B. Jonnalagadda
• Published 3 April 2018
• Chemistry, Biology
• Journal of Environmental Science and Health, Part B

ABSTRACT Ficus burtt-davyi (Moraceae) is a medicinal plant species indigenous to Southern Africa. In this study, a phytochemical and cytotoxic investigation on F. burtt-davyi was conducted to evaluate its ethno-medicinal use. The phytochemical study of the fruits yielded triterpenoids (lupeol and α-amyrin). The cytotoxic evaluation was done on the methanolic extracts and selected compounds, lupeol, α-amyrin, lupeol acetate and (+)-catechin isolated from F. burtt-davyi stem bark and fruits. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay was carried out against two human cancer cell lines, breast adenocarcinoma (MCF-7) and colorectal adenocarcinoma (Caco-2), and normal human embryonic kidney cells (HEK293). The methanol extract from the stem bark was significantly cytotoxic to MCF-7 and Caco-2 cell lines (p < 0.05) in a concentration-dependent manner with IC50 values of 6.6 and 8.1 µg mL−1, respectively relative to the control. Lupeol and (+)-catechin showed cytotoxic activity against MCF-7 cell lines with IC50 values of 22.6 and 29.8 µg mL−1, respectively and greater cytotoxic activity against Caco-2 cell lines with IC50 values of 10.7 and 9.0 µg mL−1, respectively. Data from this study suggests that F. burtt-davyi exhibits cytotoxicity with no significant inhibitory effects against HEK293. The results also indicate that (+)-catechin and lupeol, the most abundant bioactive principles in the stem bark, are responsible for the synergistic cytotoxic effects against tested human cancer cell lines. This study provides evidence on the pharmaceutical potential of the medicinal plant, F. burtt-davyi, as a chemotherapeutic agent against cancer.