Cytosolic adenylate kinase catalyzes the synthesis of thiamin triphosphate from thiamin diphosphate.

@article{Shikata1989CytosolicAK,
  title={Cytosolic adenylate kinase catalyzes the synthesis of thiamin triphosphate from thiamin diphosphate.},
  author={Hiroo Shikata and Shinya Koyama and Yuichiro Egi and Kazuko Yamada and Toshisuke Kawasaki},
  journal={Biochemistry international},
  year={1989},
  volume={18 5},
  pages={933-41}
}
An attempt was made to purify a porcine skeletal muscle enzyme catalyzing the formation of thiamin triphosphate (TTP) from thiamin diphosphate (TDP), requiring ATP, Mg2+ and a cofactor (creatine). As the purification proceeded, the reaction requirements for ATP and creatine were lost and then a requirement for ADP was manifested. The activity responsible for TTP synthesis from TDP, ADP, and Mg2+ was found to be copurified with adenylate kinase [EC 2.7.4.3] activity, and was finally purified to… CONTINUE READING
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