Although the structure of cross-linking molecules mainly determines the structural organization of actin filaments and with that the static elastic properties of the cytoskeleton, it is largely unknown how the biochemical characteristics of transiently cross-linking proteins (actin-binding proteins (ABPs)) affect the viscoelasticity of actin networks. In… (More)
FIGURE 4 (A) Frequency response of actin/HMM networks (ca ¼ 9.5 mM, R ¼ 0.1) at distinct temperatures (10 C (>) up to 30 C (6)). Solid symbols denote G0(f), open symbols denote G00(f). The solid and dashed lines represent the model used to evaluate the macromechanical response, as described in Lieleg et al. (13). (B) Plateau modulus G0 (,) and the normalized time t0/h (þ) as a function of temperature. The character h denotes the viscosity of water. (C) Temperature-oscillation protocol as described in the article. Frequency sweeps (bars) are taken at 21 C, 15 C, 25 C, 10 C, and 30 C. Before the next temperature jump is initiated, the network is brought back to its initial temperature of 21 C to assure reversibility (dots).