Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection.

@article{Ruggeri2015CytoskeletalAA,
  title={Cytoskeletal alterations associated with donor age and culture interval for equine oocytes and potential zygotes that failed to cleave after intracytoplasmic sperm injection.},
  author={Elena Ruggeri and Keith F. DeLuca and Cesare Galli and Giovanna Lazzari and Jennifer G DeLuca and Elaine M. Carnevale},
  journal={Reproduction, fertility, and development},
  year={2015},
  volume={27 6},
  pages={944-56}
}
Intracytoplasmic sperm injection (ICSI) is an established method to fertilise equine oocytes, but not all oocytes cleave after ICSI. The aims of the present study were to examine cytoskeleton patterns in oocytes after aging in vitro for 0, 24 or 48h (Experiment 1) and in potential zygotes that failed to cleave after ICSI of oocytes from donors of different ages (Experiment 2). Cytoplasmic multiasters were observed after oocyte aging for 48h (P<0.01). A similar increase in multiasters was… CONTINUE READING

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