Cytoprotection by Bcl-2 requires the pore-forming alpha5 and alpha6 helices.

  title={Cytoprotection by Bcl-2 requires the pore-forming alpha5 and alpha6 helices.},
  author={Shigemi Matsuyama and Sharon L. Schendel and Zhongwen Xie and John C. Reed},
  journal={The Journal of biological chemistry},
  volume={273 47},
We explored whether the putative channel-forming fifth and sixth alpha-helices of Bcl-2 and Bax account for Bcl-2-mediated cell survival and Bax-induced cell death in mammalian cells and in the yeast Saccharomyces cerevisiae. When alpha5-alpha6 were either deleted or swapped with each other, the Bcl-2Deltaalpha5alpha6 deletion mutant and Bcl-2-Bax(alpha5alpha6) chimeric protein failed to block apoptosis induced by either Bax or staurosporine in human cells and were unable to prevent Bax-induced… CONTINUE READING

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