Cytoplasmic orientation and two-domain structure of the multidrug transporter, P-glycoprotein, demonstrated with sequence-specific antibodies.

@article{Yoshimura1989CytoplasmicOA,
  title={Cytoplasmic orientation and two-domain structure of the multidrug transporter, P-glycoprotein, demonstrated with sequence-specific antibodies.},
  author={Akihiko Yoshimura and Y Kuwazuru and Tomoyuki Sumizawa and Masumi Ichikawa and Shun Ichi Ikeda and Taizo Uda and Saburo Akiyama},
  journal={The Journal of biological chemistry},
  year={1989},
  volume={264 27},
  pages={16282-91}
}
The predicted cytoplasmic orientation and two-domain structure of the multidrug efflux pump P-glycoprotein were demonstrated with sequence-specific antibodies. We synthesized peptides corresponding to amino acid residues, Glu393-Lys408 (anti-P) and Leu1206-Thr1226 (anti-C) in P-glycoprotein from human mdr1 cDNA and used these peptides to produce polyclonal antibodies. From the primary structure of P-glycoprotein, and anti-C antibody is expected to recognize another position, Leu561-Thr581, in… CONTINUE READING

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