Cytoplasmic metallothionein overexpression protects NIH 3T3 cells from tert-butyl hydroperoxide toxicity.

@article{Schwarz1994CytoplasmicMO,
  title={Cytoplasmic metallothionein overexpression protects NIH 3T3 cells from tert-butyl hydroperoxide toxicity.},
  author={Margaret A Schwarz and John S Lazo and Jack C. Yalowich and Ian Reynolds and Valerian E Kagan and Vladimir Tyurin and Young Myeong Kim and Simon C. Watkins and Bruce R. Pitt},
  journal={The Journal of biological chemistry},
  year={1994},
  volume={269 21},
  pages={15238-43}
}
Metallothioneins (MT) are ubiquitous low molecular weight metal-binding proteins that may act as antioxidants. We examined the sensitivity of NIH 3T3 cells transfected with a plasmid containing mouse metallothionein-I gene (NIH3T3/MT) to the membrane permeant oxidant, tert-butyl hydroperoxide (tBH). NIH3T3/MT cells had a 4-fold increase in intracellular metallothionein as compared to cells transfected with a plasmid containing an inverted gene (NIH3T3/TM). Newly expressed metallothionein… CONTINUE READING