Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA.

  title={Cytoplasmic inhibition of carotenoid biosynthesis with virus-derived RNA.},
  author={Monto H. Kumagai and Jonathan Donson and Guy della-Cioppa and David Harvey and Kathleen M. Hanley and Laurence K. Grill},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={92 5},
The carotenoid biosynthetic pathway in higher plants was manipulated by using an RNA viral vector. A cDNA encoding phytoene synthase and a partial cDNA encoding phytoene desaturase (PDS) were placed under the transcriptional control of a tobamovirus subgenomic promoter. One to two weeks after inoculation, systemically infected Nicotiana benthamiana plants were analyzed for phytoene. Leaves from transfected plants expressing phytoene synthase developed a bright orange phenotype and accumulated… CONTINUE READING
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