Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation

@article{Howell2001CytoplasmicDD,
  title={Cytoplasmic dynein/dynactin drives kinetochore protein transport to the spindle poles and has a role in mitotic spindle checkpoint inactivation},
  author={B. Howell and B. F. McEwen and J. C. Canman and D B Hoffman and E. M. Farrar and C L Rieder and E. D. Salmon},
  journal={The Journal of Cell Biology},
  year={2001},
  volume={155},
  pages={1159 - 1172}
}
We discovered that many proteins located in the kinetochore outer domain, but not the inner core, are depleted from kinetochores and accumulate at spindle poles when ATP production is suppressed in PtK1 cells, and that microtubule depolymerization inhibits this process. These proteins include the microtubule motors CENP-E and cytoplasmic dynein, and proteins involved with the mitotic spindle checkpoint, Mad2, Bub1R, and the 3F3/2 phosphoantigen. Depletion of these components did not disrupt… CONTINUE READING