Cytoplasmic assembly and selective nuclear import of Arabidopsis Argonaute4/siRNA complexes.

@article{Ye2012CytoplasmicAA,
  title={Cytoplasmic assembly and selective nuclear import of Arabidopsis Argonaute4/siRNA complexes.},
  author={Ruiqiang Ye and Wei Wang and Taichiro Iki and Chang Liu and Yang Wu and Masayuki Ishikawa and Xueping Zhou and Yijun Qi},
  journal={Molecular cell},
  year={2012},
  volume={46 6},
  pages={859-70}
}
In plants, DNA methylation can be mediated by a class of Argonaute4 (AGO4)-associated heterochromatic siRNAs (hc-siRNAs), through a pathway termed RNA-directed DNA methylation (RdDM). It has been thought that RdDM is solely a nuclear process, as both the biogenesis and functioning of hc-siRNAs take place in the nucleus. In this study, we unexpectedly found that hc-siRNAs are predominantly present in the cytoplasm. We demonstrated that AGO4 is loaded with hc-siRNAs in the cytoplasm and the… CONTINUE READING