Cytoplasmic and nuclear retained DMPK mRNAs are targets for RNA interference in myotonic dystrophy cells.

@article{Langlois2005CytoplasmicAN,
  title={Cytoplasmic and nuclear retained DMPK mRNAs are targets for RNA interference in myotonic dystrophy cells.},
  author={Marc-Andr{\'e} Langlois and Christelle Boniface and Gang Wang and Jessica Alluin and Paul M. Salvaterra and Jack Puymirat and John J. Rossi and Nan Sook Lee},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 17},
  pages={16949-54}
}
Small interfering RNA (siRNA) duplexes induce the specific cleavage of target RNAs in mammalian cells. Their involvement in down-regulation of gene expression is termed RNA interference (RNAi). It is widely believed that RNAi predominates in the cytoplasm. We report here the co-existence of cytoplasmic and nuclear RNAi phenomena in primary human myotonic dystrophy type 1 (DM1) cells by targeting myotonic dystrophy protein kinase (DMPK) mRNAs. Heterozygote DM1 myoblasts from a human DM1 fetus… CONTINUE READING