Cytoplasmic and extracellular expression of pharmaceutical-grade mycobacterial 65-kDa heat shock protein in Lactococcus lactis.

@article{Azevedo2012CytoplasmicAE,
  title={Cytoplasmic and extracellular expression of pharmaceutical-grade mycobacterial 65-kDa heat shock protein in Lactococcus lactis.},
  author={Marcela Santiago Pacheco de Azevedo and Clarissa Santos Rocha and N Electo and Daniela Santos Pontes and Jeanne Blanco de Molfetta and Evonnildo D Costa Gonçalves and Vasco Azevedo and C{\'e}lio Lopes Silva and Anderson Miyoshi},
  journal={Genetics and molecular research : GMR},
  year={2012},
  volume={11 2},
  pages={
          1146-57
        }
}
Lactic acid bacteria (LAB) are an attractive and safe alternative for the expression of heterologous proteins, as they are nonpathogenic and endotoxin-free organisms. Lactococcus lactis, the LAB model organism, has been extensively employed in the biotechnology field for large-scale production of heterologous proteins, and its use as a "cell factory" has been widely studied. We have been particularly interested in the use of L. lactis for production of heat shock proteins (HSPs), which… CONTINUE READING

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