Cytoplasmic actomyosin fibrils in tissue culture cells: direct proof of contractility by visualization of ATP-induced contraction in fibrils isolated by laser micro-beam dissection.

@article{Isenberg1976CytoplasmicAF,
  title={Cytoplasmic actomyosin fibrils in tissue culture cells: direct proof of contractility by visualization of ATP-induced contraction in fibrils isolated by laser micro-beam dissection.},
  author={Gerhard Isenberg and P C Rathke and Norbert H{\"u}lsmann and W W Franke and Karl-Ernst Wohlfarth-Bottermann},
  journal={Cell and tissue research},
  year={1976},
  volume={166 4},
  pages={427-43}
}
A special cell line derived from a rat mammary adenocarcinoma (RMCD cells) displays a distinct pattern of actomyosin fibrils (AM fibrils) visible with phase contrast, Nomarski interference and polarized light optics. It was shown that the cytoplasmic AM fibrils are arranged as bundles of highly parallel F-actin filaments. The chimical nature of the filaments was identified by incubation with heavy meromyosin from rabbit skeletal muscle. These cytoplasmic actomyosin fibrils actively contract… CONTINUE READING

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