Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture.

Abstract

Cell-to-cell interactions of human mesenchymal multipotent stromal cells (MMSC) and rat renal tubular cells (RTC) were explored under conditions of co-cultivation. We observed formation of different types of intercellular contacts, including so called tunneling nanotubes. These contacts were shown to be able to provide transfer of cell's contents, including organelles. We documented intercellular exchange with fluorescent probes specific to cytosol, plasmalemma and mitochondria. Initial transport of cellular components was revealed after 3 h of co-culturing, and occurred in two directions--both direct and retrograde as referred to RTC. However, transport of probes toward MMSC was more efficient. One significant result of such transport was appearance of renal-specific Tamm-Horsfall protein in MMSC, indicating induction of their differentiation into kidney tubular cells. We conclude that transfer of cellular compartments between renal and stem cells could provide differentiation of MMSC when transplanted into kidney and result in therapeutic benefits in renal failure.

DOI: 10.1016/j.yexcr.2010.06.009
02040602011201220132014201520162017
Citations per Year

135 Citations

Semantic Scholar estimates that this publication has 135 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Plotnikov2010CytoplasmAO, title={Cytoplasm and organelle transfer between mesenchymal multipotent stromal cells and renal tubular cells in co-culture.}, author={Egor Yu Plotnikov and Tatyana G Khryapenkova and Svetlana I Galkina and Gennady T. Sukhikh and Dmitry B. Zorov}, journal={Experimental cell research}, year={2010}, volume={316 15}, pages={2447-55} }