Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus.

@article{Moser1999CytopathogenicAN,
  title={Cytopathogenic and noncytopathogenic RNA replicons of classical swine fever virus.},
  author={Christian Moser and Peter Stettler and Jon Duri Tratschin and Martin Hofmann},
  journal={Journal of virology},
  year={1999},
  volume={73 9},
  pages={7787-94}
}
To determine the minimal requirements for autonomous RNA replication of classical swine fever virus (CSFV), genomes having in-frame deletions within the genes for structural and flanking nonstructural proteins were constructed, based on an infectious cDNA clone of CSFV Alfort/187. RNA was transcribed in vitro from the respective plasmids and transfected into SK-6 swine kidney cells. The replication competence of the RNA was determined by immunostaining transfected cells for CSFV NS3 protein and… CONTINUE READING