Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage.

@article{Huang2006CytometricAO,
  title={Cytometric assessment of histone H2AX phosphorylation: a reporter of DNA damage.},
  author={Xuan Huang and Zbigniew Darzynkiewicz},
  journal={Methods in molecular biology},
  year={2006},
  volume={314},
  pages={
          73-80
        }
}
DNA damage that leads to formation of DNA double-strand breaks (DSBs) induces phosphorylation of histone H2AX on Ser-139 at sites flanking the breakage. Immunocytochemical detection of phosphorylated H2AX (denoted as gammaH2AX) thus provides a marker of DSBs. The method presented in this chapter describes the detection of gammaH2AX for revealing the presence of DSBs, combined with differential staining of cellular DNA for revealing the cell cycle phase. The detection of gammaH2AX is based on… CONTINUE READING
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